研究開発・学会発表

Objective: To evaluate whether stimulation of endometrium embryo transfer (SEET) can improve implantation
rate and pregnancy rate (PR) for patients undergoing assisted reproductive technology (ART) for the first time
by injecting embryo culture supernatant into the uterus before blastocyst transfer (BT).
Design: Randomized, controlled trial.
Setting: Private in vitro fertilization clinic.
Patient(s): Forty-eight women in the BT group, 48 women in the stimulation group who had culture medium
injected into the uterus before BT, and 48 women in the SEET group.
Intervention(s): Injection of embryo culture supernatant and injection of culture medium.
Main Outcome Measure(s): Implantation rates and PRs.
Result(s): Odds ratios of successful implantation rate for stimulation and SEET in patients with high-grade blastocysts,
havingBTas reference, were 2.58 and 6.46 without adjustment , and 5.91 and 9.20 after adjusting for basalFSH
levels and period of infertility. Odds ratios of clinical pregnancies were 2.47 and 4.32 without adjustment, and 4.46 and 5.10 with adjustment, respectively. In groups with low-grade blastocysts, such tendencies were not observed.
Conclusion(s): The SEET may be an effective method for increasing implantation rate and PR for first-time ART
patients who have a high-grade blastocyst. (Fertil Steril 2009;92:1264–8. 2009 by American Society for Reproductive Medicine.)
Key Words: SEET, blastocyst transfer, embryo culture supernatant, cross-talk