研究開発・学会発表
診療・治療
American Society for Reproductive Medicine 65th Annual Meeting
- The role of vascular endothelial growth factor in ischemia-reperfusion injury of testis
- October 17-21, 2009 Georgia World Congress Center Atlanta, Georgia
- American Society for Reproductive Medicine 65th Annual Meeting
- Hiromi Hashimoto1,2, Tomomoto Ishikawa2, Kohei Yamaguchi2, Koji Chiba2, Masahide Shiotani1, Masato Fujisawa2
1Reproduction center, Hanabusa Women's Clinic, 1-1-2,Sannomiya Central Building, Sannomiyacho, Chuoku Kobe, Hyogo, Japan, 650-0021 and 2Urology, Kobe Univercity faculty of medicine, 7-5-1,Kusunokicho Chuoku Kobe, Hyogo, Japan, 650-0017.
【発表の概要】
Objective Testicular torsion causes ischemia-reperfusion injury of testis and leads to male factor infertility. Its injury initiates a pathophysiologic cascade. In recent studies, protective effect of VEGF has been shown in various forms of ischemia- reperfusion injury. However, no study has investigated the role of VEGF in testicular ischemia-reperfusion injury. The aim of our study was to investigate the time course expression of VEGF, VEGF-receptor (R)1, VEGF-R2, inducible and endothelial nitric oxide synthase (iNOS and eNOS) and to evaluate the effect of VEGF in ischemia-reperfusion injury of testis.
Design Animal study
Materials and Methods Using 8-week-old SD rat, surgery for making unilateral experimental torsion was performed. After 1 hour of torsion, the testis was counter-rotated back to the natural position and reinserted into the scrotum. Bilateral castrations were performed in various time courses after reperfusion. Western bolt analysis and immunohistochemical evaluation were used to determine the activation of VEGF, iNOS, and eNOS. VEGF, VEGF-R1 and -R2 mRNAs were measured by reverse transcription PCR (RT-PCR) and quantitative real-time PCR (QRT-PCR) analysis.
Results In torsion side testis, the expression of VEGF protein and mRNA significantly increased in a time dependent manner since 3 to 120 hour after reperfusion. Although the expression of VEGF-R1 mRNA was increased in a similar way, which of VEGF-R2 mRNA could not be detected. The increase of VEGF protein expression was observed in testicular vascular endothelial cells and seminiferous tubular endothelial cells. Significant activation of iNOS and eNOS were investigated at 12 and 24 hours after reperfusion.
Conclusions Our study is the first report in the literature to show the time course expression of VEGF in torsion caused ischemia-reperfusion injury testis. Further investigations, such as VEGF infusion study, may lead to a novel approach for the therapy of ischemia-reperfusion injury of the testis.
Support None.
